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Distribution of Golgi bodies (red) during mitosis of a multinucleated hyphal tip cell of Aspergillus nidulans.
- Green muclei reflect the subcellular localization of a protein fusion between PacC27 and GFP. Note that the Golgi is not dissasembled during mitosis. Details may be found in Pantazopoulou and Peñalva (2009) Mol. Biol. Cell 20:4335-4347. The pH signal transduction pathway involves endocytic membrane trafficking and is regulated at the level of nucleocytoplasmic transport. Filamentous fungal cells represent an extreme example of polarization and are coenocytic, with several evenly spaced nuclei per cell. Thus, the hyphal cellular organization imposes spatial and temporal requirements on signal transduction pathways whose analysis in a lower eukaryote amenable to relatively easy genetic manipulation is of intrinsic interest. Contributed by Dr. Miguel Peñalva, 5 April 2010
Spindle elongation and chromosome segregation
- This movie shows spindle elongation and chromosome segregation in A. nidulans using alcA-CFP-H2A and alcA-GFP-TubA fusions. The strain used for this movie was described in Su W, et al. (2004) Eukaryot Cell 3(2):553-6. Contributed by Dr. Xin Xiang, 30 April, 2010
In vivo analysis of mitochondrial movement in Aspergillus nidulans
Nuclear migration in Aspergillus nidulans wild-type strain carrying GFP labeled nuclei
- Suelmann, R. & Fischer R. (1997) Mol. Microbiol. 25(4):757-769. Contributed by Dr. Reinhard Fischer, 4 May 2010
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Nuclear migration in growing germlings of Aspergillus nidulans
Nuclear migration in growing germlings of Aspergillus nidulans
Movement of Aspergillus nidulans microtubule plus ends, visualized by the kinesin KipA
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