Aspergillus Strains

     These lists and lineage diagrams have been compiled by AspGD curators to serve as a brief reference to some of the more commonly used laboratory strains of various Aspergillus species, rather than a comprehensive resource. The strain names on each species list below link to short summary descriptions, notes and citations. If you would like to suggest additions, corrections, or updates to this list, please send a message to AspGD curators with details.
Help






 
A. nidulans
	lineage diagram
 
 



FGSC A4 (Glasgow wild-type)

Genotype: Wild-type, veA+

Notes: The wild-type reference strain sequenced in a collaboration between Monsanto and the Broad Institute. *Please note that there is an unresolved discrepancy: the actual DNA sequence of this strain from the sequencing project contains the veA1 mutant allele.

Back to top


GR5 (FGSC A773)

Genotype: pyrG89; wA3; pyroA4; veA1

Notes: A British A4 Glasgow derivative used for constructing gene knockouts.

Hoffmann B, Eckert SE, Krappmann S, Braus GH. Sexual diploids of Aspergillus nidulans do not form by random fusion of nuclei in the heterokaryon. Genetics. 2001 Jan;157(1):141-7.
   

Back to top


TN02A3 (FGSC A1149)

Genotype: pyrG89; argB2 delta-nkuA::argB; pyroA4 veA1

Notes: Uracil auxotrophic and nkuA deletion strain used for transformation and efficient construction of gene knockouts.

Hoffmann B, Eckert SE, Krappmann S, Braus GH. Sexual diploids of Aspergillus nidulans do not form by random fusion of nuclei in the heterokaryon. Genetics. 2001 Jan;157(1):141-7.
   

Back to top


TNO2A25 (FGSC A1147)

Genotype: pyrG89, argB2, pabaB22, nkuA::argB, riboB2 veA1

Notes: A nkuA deletion strain, TN02A25 lacks the KU70 gene and thus reduces non-homologous end-joining of DNA and increases the frequency of homologous recombination, a phenotype that greatly facilitates the construction of gene knockouts.

Nayak T, Szewczyk E, Oakley CE, Osmani A, Ukil L, Murray SL, Hynes MJ, Osmani SA, Oakley BR. A versatile and efficient gene-targeting system for Aspergillus nidulans. Genetics. 2006 Mar;172(3):1557-66. Epub 2005 Dec 30.
   

Back to top





 
A. fumigatus
	lineage diagram
 
 



Af293 (FGSC A1100, IHEM18963)

Genotype: Wild-type, MAT1-2

Notes: Clinical strain isolated by by David Denning from a lung biopsy from a neutropenic patient with human lung invasive aspergillosis (IA). This strain has been used as a reference strain for the A. fumigatus genome sequencing effort by TIGR, the Sanger Centre, and the Institute Pasteur with major funding provided by the National Institute of Allergy and Infectious Diseases (NIAID), the Wellcome Trust and the Fondo de Investigaciones Sanitarias. It has a 29.4-megabase genome consisting of eight chromosomes, with 9,926 predicted genes.

Nierman WC, Pain A, Anderson MJ, Wortman JR, Kim HS, Arroyo J, Berriman M, Abe K, Archer DB, Bermejo C, Bennett J, Bowyer P, Chen D, Collins M, Coulsen R, Davies R, Dyer PS, Farman M, Fedorova N, Fedorova N, Feldblyum TV, Fischer R, Fosker N, Fraser A, Garcia JL, García MJ, Goble A, Goldman GH, Gomi K, Griffith-Jones S, Gwilliam R, Haas B, Haas H, Harris D, Horiuchi H, Huang J, Humphray S, Jimenez J, Keller N, Khouri H, Kitamoto K, Kobayashi T, Konzack S, Kulkarni R, Kumagai T, Lafon A, Latge JP, Li W, Lord A, Lu C, Majoros WH, May GS, Miller BL, Mohamoud Y, Molina M, Monod M, Mouyna I, Mulligan S, Murphy L, O'Neil S, Paulsen I, Penalva MA, Pertea M, Price C, Pritchard BL, Quail MA, Rabbinowitsch E, Rawlins N, Rajandream MA, Reichard U, Renauld H, Robson GD, Rodriguez de Cordoba S, Rodríguez-Pena JM, Ronning CM, Rutter S, Salzberg SL, Sanchez M, Sanchez-Ferrero JC, Saunders D, Seeger K, Squares R, Squares S, Takeuchi M, Tekaia F, Turner G, Vazquez de Aldana CR, Weidman J, White O, Woodward J, Yu JH, Fraser C, Galagan JE, Asai K, Machida M, Hall N, Barrell B, Denning DW. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature. 2005 Dec 22;438(7071):1151-6.
   

Back to top


Af293.1

Genotype: pyrG-, MAT1-2

Notes: A pyrG1 mutant derivative of the wild-type clinical isolate and reference sequence strain, Af293. This uracil auxotroph strain was derived by selection of A. fumigatus AF293 on MAG solid medium supplemented with 5 mM uracil, 10 mM uridine and 1 mg/mL 5-fluoroorotic acid (5-FOA) following mutagenesis of conidia with 4-nitroquinoline 1-oxide.

Osherov N, Kontoyiannis DP, Romans A, May GS. Resistance to itraconazole in Aspergillus nidulans and Aspergillus fumigatus is conferred by extra copies of the A. nidulans P-450 14alpha-demethylase gene, pdmA. J Antimicrob Chemother. 2001 Jul;48(1):75-81.
   

Xue T, Nguyen CK, Romans A, Kontoyiannis DP, May GS. Isogenic auxotrophic mutant strains in the Aspergillus fumigatus genome reference strain AF293. Arch Microbiol. 2004 Nov;182(5):346-53. Epub 2004 Sep 9.
   

Back to top


CEA10 (CBS144.89, FGSC A1163, AF10)

Genotype: Wild type, MAT1-1

Notes: Isolated from an invasive aspergillosis patient. Sequenced by the J. Craig Venter Institute, in collaboration with Celera Genomics. Its genome is 29.2 Mb in size.

Fedorova ND, Khaldi N, Joardar VS, Maiti R, Amedeo P, Anderson MJ, Crabtree J, Silva JC, Badger JH, Albarraq A, Angiuoli S, Bussey H, Bowyer P, Cotty PJ, Dyer PS, Egan A, Galens K, Fraser-Liggett CM, Haas BJ, Inman JM, Kent R, Lemieux S, Malavazi I, Orvis J, Roemer T, Ronning CM, Sundaram JP, Sutton G, Turner G, Venter JC, White OR, Whitty BR, Youngman P, Wolfe KH, Goldman GH, Wortman JR, Jiang B, Denning DW, Nierman WC. Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. PLoS Genet. 2008 Apr 11;4(4):e1000046.
   

Girardin H, Latge JP, Srikantha T, Morrow B, Soll DR. Development of DNA probes for fingerprinting Aspergillus fumigatus. J Clin Microbiol. 1993 Jun;31(6):1547-54.
   

Back to top


CEA17 (FGSC 1152)

Genotype: pyrG-, MAT1-1

Notes: Strain CEA17 is a pyrG1 derivative of strain CEA10/FGSC A1163. It is a uracil and uridine auxotroph.

Fedorova ND, Khaldi N, Joardar VS, Maiti R, Amedeo P, Anderson MJ, Crabtree J, Silva JC, Badger JH, Albarraq A, Angiuoli S, Bussey H, Bowyer P, Cotty PJ, Dyer PS, Egan A, Galens K, Fraser-Liggett CM, Haas BJ, Inman JM, Kent R, Lemieux S, Malavazi I, Orvis J, Roemer T, Ronning CM, Sundaram JP, Sutton G, Turner G, Venter JC, White OR, Whitty BR, Youngman P, Wolfe KH, Goldman GH, Wortman JR, Jiang B, Denning DW, Nierman WC. Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. PLoS Genet. 2008 Apr 11;4(4):e1000046.
   

d'Enfert C. Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker. Curr Genet. 1996 Jun;30(1):76-82.
   

Back to top


KU80-delta pyrG1 (FGSC A1160)

Genotype: akuB (KU80)-delta pyrG1 MAT1-1

Notes: This strain was made by transformation of strain CEA17 with an akuB deletion construct. This strain displays an increase in the frequency of homologous recombination, a phenotype that facilitates the construction of gene knockouts.

da Silva Ferreira ME, Kress MR, Savoldi M, Goldman MH, Hartl A, Heinekamp T, Brakhage AA, Goldman GH. The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryot Cell. 2006 Jan;5(1):207-11.
   

Back to top


ATCC46645

Genotype: Wild-type,MAT1-1

Notes: A wild-type clinical strain isolated from a human infection.

Olias P, Gruber AD, Hafez HM, Lierz M, Slesiona S, Brock M, Jacobsen ID. Molecular epidemiology and virulence assessment of Aspergillus fumigatus isolates from white stork chicks and their environment. Vet Microbiol. 2011 Mar 24;148(2-4):348-55. Epub 2010 Sep 8.
   

Langfelder K, Jahn B, Gehringer H, Schmidt A, Wanner G, Brakhage AA. Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. Med Microbiol Immunol. 1998 Oct;187(2):79-89.
   

Verweij PE, Oakley KL, Morrissey J, Morrissey G, Denning DW. Efficacy of LY303366 against amphotericin B-susceptible and -resistant Aspergillus fumigatus in a murine model of invasive aspergillosis. Antimicrob Agents Chemother. 1998 Apr;42(4):873-8.
   

De Lucas JR, Dominguez AI, Higuero Y, Martinez O, Romero B, Mendoza A, Garcia-Bustos JF, Laborda F. Development of a homologous transformation system for the opportunistic human pathogen Aspergillus fumigatus based on the sC gene encoding ATP sulfurylase. Arch Microbiol. 2001 Jul;176(1-2):106-13.
   

Back to top


akuA::loxP (FGSC 1159)

Genotype: akuA::loxP, MAT1-1

Notes: An akuA::loxP derivative of the clinical isolate ATCC 46645.

Hartmann T, Dumig M, Jaber BM, Szewczyk E, Olbermann P, Morschhauser J, Krappmann S. Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system. Appl Environ Microbiol. 2010 Sep;76(18):6313-7. Epub 2010 Jul 23.
   

Back to top


akuA::ptrA (FGSC 1157)

Genotype: akuA::ptrA

Notes: An akuA::ptrA strain that has an increased frequency of homologous integration, a phenotype that greatly facilitates the construction of gene knockouts.

Krappmann S, Sasse C, Braus GH. Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background. Eukaryot Cell. 2006 Jan;5(1):212-5.
   

Back to top


akuA::loxP-hygro-R/tk (FGSC 1158)

Genotype:akuA::loxP

Notes: A derivitive of akuA::ptrA (FGSC 1157) that has the loxP-hph/tk marker integrated at the atrA::ptrA locus.

Krappmann S, Sasse C, Braus GH. Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background. Eukaryot Cell. 2006 Jan;5(1):212-5.
   

Back to top


Af210

Genotype: Clinical isolate, presumably wild type

Notes: An amphotericin B-susceptible clinical isolate that was cultured from the abdominal viscera from a surgical patient who developed an Aspergillus wound infection following laparotomy and who responded to conventional amphotericin B treatment without change of immune status.

Carlson GL, Mughal MM, Birch M, Denning DW. Aspergillus wound infection following laparostomy. J Infect. 1996 Sep;33(2):119-21.
   

Back to top





 
A. niger
	lineage diagram
 
 



CBS 513.88

Genotype:MAT1-1

Notes: Industrial enzyme-producing A. niger strain that was sequenced by Integrated Genomics. It is a derivative of NRRL 3122 (ATCC 22343, CBS 115989) that was isolated after mutagenesis and selection for improved glucoamylase production.

Baker SE. Aspergillus niger genomics: past, present and into the future. Med Mycol. 2006 Sep;44 Suppl 1:S17-21.
   

Pal K, van Diepeningen AD, Varga J, Hoekstra RF, Dyer PS, Debets AJ. Sexual and vegetative compatibility genes in the aspergilli. Stud Mycol. 2007;59:19-30.
   

Back to top


ATCC 1015 (NRRL 328, CBS 113.46)

Genotype: Wild-type, MAT1-1

Notes: Acidogenic wild-type strain. Reference strain sequenced in a collaboration between the US Department of Energy, the Joint Genome Institute and the Pacific Northwest National Fungal Biotechnology Team.

Andersen MR, Salazar MP, Schaap PJ, van de Vondervoort PJ, Culley D, Thykaer J, Frisvad JC, Nielsen KF, Albang R, Albermann K, Berka RM, Braus GH, Braus-Stromeyer SA, Corrochano LM, Dai Z, van Dijck PW, Hofmann G, Lasure LL, Magnuson JK, Menke H, Meijer M, Meijer SL, Nielsen JB, Nielsen ML, van Ooyen AJ, Pel HJ, Poulsen L, Samson RA, Stam H, Tsang A, van den Brink JM, Atkins A, Aerts A, Shapiro H, Pangilinan J, Salamov A, Lou Y, Lindquist E, Lucas S, Grimwood J, Grigoriev IV, Kubicek CP, Martinez D, van Peij NN, Roubos JA, Nielsen J, Baker SE. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 2011 Jun;21(6):885-97. Epub 2011 May 4.
   

Pal K, van Diepeningen AD, Varga J, Hoekstra RF, Dyer PS, Debets AJ. Sexual and vegetative compatibility genes in the aspergilli. Stud Mycol. 2007;59:19-30.
   

Back to top


ATCC 1015 pyrG-delta MAT1-1

Notes: A pyrG auxotrophic derivative of the acidogenic wild-type strain reference strain ATCC 1015. Used for the construction gene deletion strains.

Mojzita D, Wiebe M, Hilditch S, Boer H, Penttila M, Richard P. Metabolic engineering of fungal strains for conversion of D-galacturonate to meso-galactarate. Appl Environ Microbiol. 2010 Jan;76(1):169-75. Epub 2009 Nov 6.
   

Back to top


NRRL 3 (N400, ATCC 9029, CBS 120.49)

Genotype: Wild-type

Notes: Wild-type strain, sequenced by Integrated Genomics.

Bos CJ, Debets AJ, Swart K, Huybers A, Kobus G, Slakhorst SM. Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger. Curr Genet. 1988 Nov;14(5):437-43.
   

Back to top


N402 (ATCC 64974)

Genotype: cspAl

Notes: A cspAl derivative of N400/ATCC 9029. Used for mitotic recombination and genetic mapping studies.

Bos CJ, Debets AJ, Swart K, Huybers A, Kobus G, Slakhorst SM. Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger. Curr Genet. 1988 Nov;14(5):437-43.
   

Back to top


AB4.1

Genotype: pyrG-

Notes: A pyrG derivative of strain N402/ATCC 64974.

van Hartingsveldt W, Mattern IE, van Zeijl CM, Pouwels PH, van den Hondel CA. Development of a homologous transformation system for Aspergillus niger based on the pyrG gene. Mol Gen Genet. 1987 Jan;206(1):71-5.
   

Back to top





 
A. oryzae
	lineage diagram
 
 



RIB40 (ATCC 42149)

Genotype: Wild-type, MATalpha

Notes: Sequenced strain of A. oryzae. This strain was solated in 1950 from cereal (raw material of shoyu) in Kyoto, Japan. This strain, which is known to produce much amount of hydrolases as well as organic acid and pigment, has an ability to produce both sake and shoyu. RIB40 is a cyclopiazonic acid (CPA) nonproducing strain. It does not biosynthesize cyclo-acetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene.

Machida M, Asai K, Sano M, Tanaka T, Kumagai T, Terai G, Kusumoto K, Arima T, Akita O, Kashiwagi Y, Abe K, Gomi K, Horiuchi H, Kitamoto K, Kobayashi T, Takeuchi M, Denning DW, Galagan JE, Nierman WC, Yu J, Archer DB, Bennett JW, Bhatnagar D, Cleveland TE, Fedorova ND, Gotoh O, Horikawa H, Hosoyama A, Ichinomiya M, Igarashi R, Iwashita K, Juvvadi PR, Kato M, Kato Y, Kin T, Kokubun A, Maeda H, Maeyama N, Maruyama J, Nagasaki H, Nakajima T, Oda K, Okada K, Paulsen I, Sakamoto K, Sawano T, Takahashi M, Takase K, Terabayashi Y, Wortman JR, Yamada O, Yamagata Y, Anazawa H, Hata Y, Koide Y, Komori T, Koyama Y, Minetoki T, Suharnan S, Tanaka A, Isono K, Kuhara S, Ogasawara N, Kikuchi H. Genome sequencing and analysis of Aspergillus oryzae. Nature. 2005 Dec 22;438(7071):1157-61.
   

Galagan JE, Calvo SE, Cuomo C, Ma LJ, Wortman JR, Batzoglou S, Lee SI, Basturkmen M, Spevak CC, Clutterbuck J, Kapitonov V, Jurka J, Scazzocchio C, Farman M, Butler J, Purcell S, Harris S, Braus GH, Draht O, Busch S, D'Enfert C, Bouchier C, Goldman GH, Bell-Pedersen D, Griffiths-Jones S, Doonan JH, Yu J, Vienken K, Pain A, Freitag M, Selker EU, Archer DB, Penalva MA, Oakley BR, Momany M, Tanaka T, Kumagai T, Asai K, Machida M, Nierman WC, Denning DW, Caddick M, Hynes M, Paoletti M, Fischer R, Miller B, Dyer P, Sachs MS, Osmani SA, Birren BW. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature. 2005 Dec 22;438(7071):1105-15.
   

Scazzocchio C. Aspergillus genomes: secret sex and the secrets of sex. Trends Genet. 2006 Oct;22(10):521-5. Epub 2006 Sep 5.
   

Tamano K, Sano M, Yamane N, Terabayashi Y, Toda T, Sunagawa M, Koike H, Hatamoto O, Umitsuki G, Takahashi T, Koyama Y, Asai R, Abe K, Machida M. Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation. Fungal Genet Biol. 2008 Feb;45(2):139-51. Epub 2007 Sep 25.
   

Tokuoka M, Seshime Y, Fujii I, Kitamoto K, Takahashi T, Koyama Y. Identification of a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene required for the biosynthesis of cyclopiazonic acid in Aspergillus oryzae. Fungal Genet Biol. 2008 Dec;45(12):1608-15. Epub 2008 Sep 27.
   

Back to top


niaD300 (NSR13)

Genotype: niaD- MATalpha

Jin FJ, Maruyama J, Juvvadi PR, Arioka M, Kitamoto K. Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae. FEMS Microbiol Lett. 2004 Oct 1;239(1):79-85.
   

Yamada O, Lee BR, Gomi, K. Transformation system for Aspergillus oryzae with double auxotrophic mutations, niaD and sC. Biosci. Biotechnol. Biochem., 61 (1997), pp. 1367-1369.
 

Back to top


NS4

Genotype: niaD-, sC-, MATalpha

Notes: A sC- mutant derivative of A. oryzae strain niaD300/NSR13.

Jin FJ, Maruyama J, Juvvadi PR, Arioka M, Kitamoto K. Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae. FEMS Microbiol Lett. 2004 Oct 1;239(1):79-85.
   

Back to top


NSRKu70-1-1 (NSRKu70-1-1A)

Genotype: niaD- sC-adeA- delta-argB::adeA delta-ku70::argB

Notes: Auxotrophic mutant used for transformation and gene knockout experiments.

Escano CS, Juvvadi PR, Jin FJ, Takahashi T, Koyama Y, Yamashita S, Maruyama J, Kitamoto K. Disruption of the Aopex11-1 gene involved in peroxisome proliferation leads to impaired Woronin body formation in Aspergillus oryzae. Eukaryot Cell. 2009 Mar;8(3):296-305. Epub 2009 Jan 9.
   

Back to top


RkuN16ptr1

Genotype: delta-ku70::ptrA delta-pyrG

Notes: Due to the absence of Ku70, this A. oryzae strain displays an increased frequency of homologous recombination, a phenotype that greatly facilitates the construction of gene knockouts.

Takahashi T, Masuda T, Koyama Y. Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae. Mol Genet Genomics. 2006 May;275(5):460-70. Epub 2006 Feb 10.
   

Back to top





If you would like to suggest additions or updates to this list, please send a message to AspGD curators with details.

Return to AspGD Send a Message to the AspGD Curators