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Liu HL, Osmani AH, Ukil L, Son S, Markossian S, Shen KF, Govindaraghavan M, Varadaraj A, Hashmi SB, De Souza CP, Osmani SA (2010) Single-step affinity purification for fungal proteomics. Eukaryot Cell 9(5):831-3
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Abstract:A single-step protein affinity purification protocol is described using Aspergillus nidulans. Detailed protocols are provided for cell breakage, affinity purification and, depending on the application, methods for protein release from affinity beads. Examples are provided defining the utility of the approaches which should be widely applicable.
Status:PublishedType:Journal ArticlePubMed ID:20363899

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Aspergillus nidulans/Emericella nidulans
Techniques and Reagents
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